TERF1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB15106
unconjugated
Application Notes
Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/200 - 1/1000, This product requires antigenretrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Western Blot: detects a band of approximately 60 kDa in Jurkat whole cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage. TRF1 is thought to play a role in leukaemia being expressed at significantly higher levels in patients with acute lymphoblastic leukaemia than those with myeloid leukaemia.Synonyms: NIMA-interacting protein 2, PIN2, TRBF1, TRF, TRF1, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, Telomeric repeat-binding factor 1