VAMP2 antibody (N-Term)

Catalog No. ABIN317061

The Mouse Monoclonal anti-VAMP2 antibody (Clone 3-00E-005) (ABIN317061) specifically detects VAMP2 in WB, IHC (p) and EIA. The antibody is reactive with Human and Rat samples.

Quick Overview for VAMP2 antibody (N-Term) (ABIN317061)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3-00E-005

Product Details anti-VAMP2 Antibody

Binding Specificity: AA 1-89, N-Term

Specificity: This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.

Characteristics: Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP-2 / Synaptobrevin-2

Background: Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2

Gene ID: 6844

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport