VAMP2 antibody (AA 1-89)

Catalog No. ABIN306417

The Mouse Monoclonal anti-VAMP2 antibody (Clone 3E5) (ABIN306417) specifically detects VAMP2 in WB, ELISA, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for VAMP2 antibody (AA 1-89) (ABIN306417)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3E5

Product Details anti-VAMP2 Antibody

Binding Specificity: AA 1-89

Purpose: Human VAMP-2 antibody

Purification: Purified

Immunogen: Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli

Isotype: IgG1 kappa

Application Details

Application Notes: Recommended dilution 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP-2

Background: Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin 1.

NCBI Accession: NP_055047

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport