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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (RM4-5)
Host
Reactivity
Alternatives

Mouse (Murine)
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
Catalog no. ABIN317083
Quantity 25 µg  (0.5mg/ml)  (Variants)
Price 90.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Alternative name CD4
Swiss-Prot P06332
Immunogen BALB/c mouse thymocytes
Cross-Reactivity Mouse (Murine)
Isotype IgG2a  (Matching secondary antibodies)
Clone RM4-5
Description CD4 is a single chain transmembraneous glycoprotein which belongs to theimmunoglobulin superfamily, and is primarily expressed on peripheral blood monocytesand tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells(CD4+CD25+), which play a key role in the maintenance of self tolerance.
Specificity This antibody detects CD4, a 55 kD protein also known as Ly-4 and L3T4. The RM4-5antibody is useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T celldepletions. Species: Mouse. Others not tested.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Flow Cytometry. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5mg/ml
Purification Purified
Buffer Phosphate buffered saline pH7.2 containing 0.09% Sodium Azide (NaN3)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (445), Rat (207), Rabbit (68)
Reactivities Human (321), Mouse (Murine) (238), Rat (Rattus) (106), Dog (Canine) (44), Chicken (34), Cow (Bovine) (34), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (93), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (35), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)