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Nanog Homeobox (NANOG) (AA 1-154), (N-Term) antibody

Details for Product No. ABIN317542, Supplier: Log in to see
Antigen
  • 2410002E02Rik
  • ecat4
  • ENK
Epitope
AA 1-154, N-Term
27
25
21
21
20
17
14
14
14
13
12
9
8
8
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
332
91
37
7
4
2
2
2
1
Host
Mouse
220
102
42
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
16
16
15
15
12
12
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
274
202
110
71
59
53
38
37
24
13
8
4
4
3
2
1
1
1
1
Supplier
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Immunogen Recombinant human Nanog (1-154 aa) purified from E. coli
Clone 5A10
Isotype IgG2a
Specificity The antibody recognizes NANOG.
Characteristics Synonyms: Homeobox protein NANOG
Purification Protein-G affinity chromatography
Alternative Name NANOG (NANOG Antibody Abstract)
Background Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.Synonyms: Homeobox protein NANOG
Gene ID 100293888
UniProt Q9H9S0
Application Notes ELISA. Western blot (1: 500 - 1: 2,000). Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Nanog Homeobox (NANOG) (AA 1-154), (N-Term) antibody (ABIN317542) anti-Nanog Homeobox (NANOG) (AA 1-154), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Nanog Homeobox (NANOG) (AA 1-154), (N-Term) antibody (ABIN317542) anti-Nanog Homeobox (NANOG) (AA 1-154), (N-Term) antibody (Image 2)
Background publications Tay, Zhang, Thomson, Lim, Rigoutsos: "MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation." in: Nature, Vol. 455, Issue 7216, pp. 1124-8, 2008 (PubMed).

Chiou, Yu, Huang, Lin, Liu, Tsai, Chou, Chien, Ku, Lo: "Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 13, pp. 4085-95, 2008 (PubMed).