Streptavidin antibody (AA 25-183)

Catalog No. ABIN317566

This Mouse Monoclonal antibody specifically detects Streptavidin in WB and EIA. It exhibits reactivity toward Streptomyces avidinii.

Quick Overview for Streptavidin antibody (AA 25-183) (ABIN317566)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Mouse

Clonality: Monoclonal

Conjugate: This Streptavidin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1C2

Product Details anti-Streptavidin Antibody

Binding Specificity: AA 25-183

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: StAv

Purification: Protein-G affinity chromatography

Immunogen: Recombinant Streptavidin (25-183 aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1: 1,000 - 1: 3,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Streptavidin

Target: Streptavidin

Background: Streptavidin binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.Synonyms: StAv

UniProt: P22629