STEAP1 antibody (N-Term)

Catalog No. ABIN317568

Product Details anti-STEAP1 Antibody

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Binding Specificity: AA 1-70, N-Term

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: The antibody recognizes STEAP1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Characteristics: Synonyms: STEAP-1, Metalloreductase STEAP1, PRSS24, Six-transmembrane epithelial antigen ofprostate 1

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human STEAP1 (1-70 aa) purified from E. coli

Clone: J2D2

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (cell lysates of LNcap (30 μg) and Rat testis (30 μg) were resolved bySDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1: 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system). Immunofluorescence (1: 100 - 1: 300). Immunohistochemsitry on paraffin sections (1: 50 - 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for STEAP1

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Alternative Name: STEAP / STEAP1 (STEAP1 Products)

Synonyms: PRSS24 antibody, STEAP antibody, STEAP1 antibody, 2410007B19Rik antibody, Prss24 antibody, Steap antibody, STEAP family member 1 antibody, six transmembrane epithelial antigen of the prostate 1 antibody, STEAP1 antibody, Steap1 antibody

Background: Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. NCBI Accession No: NP_036581Synonyms: Metalloreductase STEAP1, PRSS24, STEAP-1, Six-transmembrane epithelial antigen of prostate 1

Gene ID: 26872

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis