TPX2 antibody

Catalog No. ABIN317807

The Rabbit Polyclonal anti-TPX2 antibody is suitable to detect TPX2 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for TPX2 antibody (ABIN317807)

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TPX2 Antibody

Specificity: TPX2 antibody detects endogenous levels of TPX2 protein.(region surrounding Gln330)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: TPX2 / DIL2

Background: TPX2 (targeting protein for Xklp2) is a microtubule-associated protein involved in targeting the motor protein Xklp2 to microtubules. Ran-GTP activates TPX2 for the chromatin-induced microbutule assembly during M phase. Aurora-A kinase associates with TPX2 at the spindle apparatus and may regulate TPX2 via phosphorylation during the spindle assembly. TPX2 appears to play a structural role in spindle formation. TPX2 activates Eg2 in a microtubuledependent manner by stimulating the phosphorylation and kinase activity of Eg2. TPX2 is inactivated by binding to importin α, a nuclear import factor. Finally, the suppression of TPX2 with RNA interference causes defects in microtubule organization during mitosis.Synonyms: C20orf1, C20orf2, DIL-2, Differentially expressed in cancerous and non-cancerous lung cells 2, HCA519, Hepatocellular carcinoma-associated antigen 519, Restricted expression proliferation-associated protein 100, Targeting protein for Xklp2

Gene ID: 22974

NCBI Accession: NP_036244

UniProt: Q9ULW0