TPX2 antibody

Catalog No. ABIN931062

The Mouse Monoclonal anti-TPX2 antibody (Clone 3164C6a) (ABIN931062) specifically detects TPX2 in WB, IP, ICC, FACS and DB. The antibody is reactive with Human samples.

Quick Overview for TPX2 antibody (ABIN931062)

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Dot Blot (DB)

Clone: 3164C6a

Product Details anti-TPX2 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: TPX2 antibody was raised in mouse using recombinant Human Tpx2, Microtubule-Associated, Homolog (Xenopus Laevis) (Tpx2)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: TPX2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: TPX2

Background: TPX2 is a spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr 288' and protects this residue against dephosphorylation. Synonyms: Monoclonal TPX2 antibody, Anti-TPX2 antibody, Targeting protein for Xklp2 antibody, DIL2 antibody, p100 antibody, DIL-2 antibody, HCTP4 antibody, FLS353 antibody, HCA519 antibody, REPP86 antibody, C20orf1 antibody, C20orf2 antibody, GD:C20orf1 antibody.