NR4A1 antibody

Catalog No. ABIN317895

The Rabbit Polyclonal anti-NR4A1 antibody (ABIN317895) specifically detects NR4A1 in WB and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NR4A1 antibody (ABIN317895)

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR4A1 Antibody

Specificity: This antibody detects endogenous levels of Nur77/NR4A1 protein. (region surrounding Gly347)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: NAK1

Background: Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.Synonyms: Early Response Protein NAK1, GFRP1, HMR, N10, NR4A1, NUR77, Orphan Nuclear Receptor HMR, ST-59, TR3 Orphan Receptor

Molecular Weight: approx. 60 kDa

Gene ID: 3164

NCBI Accession: NP_002126

UniProt: P22736

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway