VGF antibody

Catalog No. ABIN317924

The Rabbit Polyclonal anti-VGF antibody is suitable to detect VGF in samples from Human, Rat and Mouse. It has been validated for WB and IHC (p).

Quick Overview for VGF antibody (ABIN317924)

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VGF Antibody

Specificity: VGF antibody detects endogenous levels of VGF protein.(region surrounding Lys458)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VGF

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Alternative Name: VGF

Background: Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.Synonyms: Neurosecretory protein VGF

Molecular Weight: approx. 67 kDa

Gene ID: 7425

NCBI Accession: NP_003369

UniProt: O15240

Pathways: Hormone Transport, Carbohydrate Homeostasis