VGF antibody (C-Term)

Catalog No. ABIN501116

The Rabbit Polyclonal anti-VGF antibody is suitable to detect VGF in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for VGF antibody (C-Term) (ABIN501116)

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-VGF Antibody

Binding Specificity: C-Term

Specificity: This antibody detects VGF.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography

Immunogen: VGF antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human VGF.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for VGF

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Alternative Name: VGF

Background: VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.Synonyms: Neurosecretory protein VGF

Gene ID: 7425

NCBI Accession: NP_003369

Pathways: Hormone Transport, Carbohydrate Homeostasis