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Activating Transcription Factor 2 (ATF2) (pThr71,pThr53) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Epitope
Alternatives

pThr71,pThr53
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN317966
Quantity 100 µg  (1,0 mg/ml)
Price 340.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, CyclicAMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2,cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-bindingprotein 2
Alternative name ATF2
Swiss-Prot P15336
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Description Eukaryotic gene transcription is regulated by sequence-specific transcription factors whichbind modular CIS acting promotor and enhancer elements. The cAMP response element(CRE), one of the best studied of such elements, consists of the palindromicoctanucleotide TGACGTCA. Several CRE binding proteins have been identified within theATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designatedATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucinezipper dimerization and basic DNA binding domains but are highly divergent in their aminoterminal domains. Although each of the ATF/CREB proteins appear capable of binding CREin its homodimeric form, certain of these also bind as heterodimers, both within theATF/CREB family and even with members of the AP-1 transcription factor family.
Specificity P-ATF2 (pThr71/53) antibody detects endogenous levels of p-ATF2 protein. Species: Human, Mouse, Rat. Others not tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes ELISA: 1: 20000~1: 40000. Western Blot: 1: 500~1: 1000. Immunohistochemistry: 1: 50~1: 200. Immunoprecipitation: 1: 50~1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1,0 mg/ml
Purification Aff - Purified
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Cell Cycle, Signaling, Kinases/Phosphatases
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (150), Mouse (16), Goat (4), Sheep (3)
Reactivities Human (171), Mouse (Murine) (101), Rat (Rattus) (90), Cow (Bovine) (3), Dog (Canine) (3), Pig (Porcine) (2), Rabbit (2), Horse (Equine) (1)
Applications Western Blotting (WB) (108), Immunohistochemistry (IHC) (65), Immunofluorescence (IF) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (31), ELISA (24), Flow Cytometry (FACS) (19), Dot Blot (Dot) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pThr69 or 51 (5), pThr69/51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer62/44 (4), pSer112/94 (3), pThr71/53 (3), pThr73/55 (3), S44 (2), S94 (2), S322 (1), pSer112, pSer94 (1), pSer340, pSer322 (1), pSer44 (1), pSer472 (1), pSer62, pSer44 (1), pSer94 (1), pThr51 (1), pThr69, pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79, pThr51 (1)