CTNNB1 antibody (pSer45, pThr41)

Catalog No. ABIN317978

The Rabbit Polyclonal anti-CTNNB1 antibody is suitable to detect CTNNB1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for CTNNB1 antibody (pSer45, pThr41) (ABIN317978)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CTNNB1 Antibody

Binding Specificity: pSer45, pThr41

Specificity: P-Catenin-β (pThr41+S45) antibody detects endogenous levels of p-Catenin-β protein.

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: Catenin beta-1

Background: The catenins, α, β and γ, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. β-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. β-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3β, results in accumulation of the β-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of β-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in β-catenin, inhibiting the interaction with APC, and increasing β-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of β-catenin in tumors such as breast cancer.Synonyms: Beta-catenin, CTNNB, CTNNB1

Molecular Weight: approx. 85 kDa

Gene ID: 1499

NCBI Accession: NP_001091679

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2