Nibrin antibody (pSer343)

Catalog No. ABIN318326

The Rabbit Polyclonal anti-Nibrin antibody is suitable to detect Nibrin in samples from Human and Rat. It has been validated for WB and IHC (p).

Quick Overview for Nibrin antibody (pSer343) (ABIN318326)

Target: Nibrin (NBN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nibrin Antibody

Binding Specificity: pSer343

Specificity: This antibody detects endogenous levels of Nibrin (p95/NBS1) pSer343 protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: Nibrin

Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95

Molecular Weight: approx. 95 kDa

Gene ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response