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Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) antibody

Details for Product No. ABIN319133
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Antigen
Synonyms ICE(rel)III, ICEREL-III, ICH-3, CASP5
Reactivity
Human
(79), (15), (15), (14), (11), (11), (3), (3), (3)
Host
Rabbit
(76), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(71), (32), (12), (11), (10), (10), (9), (4), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN319133
357.50 $
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Immunogen A 14 amino acid peptide from the centre of human Caspase-5.
Isotype IgG
Specificity This antibody recognises an epitope located within the internal region of human Caspase-5, a 47kDa cysteine protease, otherwise known as ICH-3.
Purification Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Alternative Name Caspase-5
Background Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).
Alternate names: CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease
Gene ID 838
NCBI Accession NP_001129581.1
UniProt P51878
Research Area Apoptosis/Necrosis
Application Notes Western Blot: 1.0g/ml - 2.0g/ml, detects a band of approximately 48 kDa in Ramos celllysates. (Predicted precursor MWT 47.8kDa)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline containing 0.02% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) antibody anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) antibody
anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) antibody (2) anti-Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) antibody (Image 2)
Background publications Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).

Wang, Miura, Jung et al.: "Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20580-7, 1996 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Lin, Choi, Porter: "Expression analysis of the human caspase-1 subfamily reveals specific regulation of the CASP5 gene by lipopolysaccharide and interferon-gamma." in: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 39920-6, 2001 (PubMed).

Validation Images
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