SNCA antibody (pSer129)

Catalog No. ABIN319236

This anti-SNCA antibody is a Rabbit Polyclonal antibody detecting SNCA in WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for SNCA antibody (pSer129) (ABIN319236)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SNCA Antibody

Binding Specificity: pSer129

Specificity: This antibody AP08013PU detects endogenous levels of alpha-Synuclein only when phosphorylated at Serine 129.

Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthetic phosphopeptide derived from Human alpha-Synuclein around the phosphorylation site of Serine 129 (M-P-SP-E-E).

Application Details

Application Notes: Western blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: alpha-Synuclein / SNCA

Background: Alpha Synuclein is implicated in the regulation of dopamine release and transport. It is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). In the nervous system, alpha Synuclein is primarily located at presynaptic terminals and is found membrane bound in dopaminergic neurons. It can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies), including Parkinson's Disease. Alpha Synuclein induces fibrillization of microtubule associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Alpha synuclein is a protein phosphorylated predominantly on serine residues.Synonyms: NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1

Gene ID: 6622

NCBI Accession: NP_000336

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity