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CD3 antibody (CD3) (FITC)

Details for Product anti-CD3 Antibody No. ABIN319654, Supplier: Log in to see
Antigen
  • CD3
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • DNA segment, Chr 9, MRC UK Mouse Genome Centre 45 expressed
  • CD3D
  • CD3E
  • D9Mgc45e
Reactivity
Human, Monkey
797
304
84
43
33
31
29
17
14
13
11
10
10
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
1
Host
Mouse
886
195
62
47
22
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3 antibody is conjugated to FITC
161
119
93
65
30
25
24
22
21
21
17
17
14
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11
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9
9
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2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
973
254
215
146
121
117
106
101
76
56
19
16
12
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11
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10
7
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4
3
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3
3
3
3
3
3
2
2
2
2
1
1
1
1
Options
Supplier
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Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Clone UCHT1
Isotype IgG1
Specificity This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19 kDa. As a pan T cell marker this antibody is present on more than 95 % of circulating human peripheral T cells.
Purification Affinity Chromatography on Protein G.
Alternative Name CD3 (CD3 Antibody Abstract)
Background CD3 is a surface protein of peripheral blood T cells of molecular weight 19 kD. The pan T cell marker is present on more than 95 % of circulating human peripheral T cells.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells or 100 μL of lysed whole peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This antibody is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Clark, Martin, Hansen, Ledbetter: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).