Ribonucleotide Reductase Inhibiting Protein SML1 antibody

Catalog No. ABIN3197499

Product Details

Target: Ribonucleotide Reductase Inhibiting Protein SML1

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known at the moment

Predicted Reactivity: Saccharomyces cerevisiae

Characteristics: Expected / apparent Molecular Weight of the Antigene: 11.83 / 11-12 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945

Application Details

Application Notes: 1 : 1000 with standard ECL (WB)

Comment:

Cell preparation for western blot: cells were harvested by centrifugation (4000 x g , 6 min, 4 °C). Supernatant was discarded and cells were resuspended in 500 µl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Complete Mini Protease inhibitor cocktail with EDTA (Roche Diagnostics GmbH)). 500 µl 0.5 mm Zirconia/Silica Beads (BioSpec Products, Inc. 11079105z) and 500 µl cold 20 % TCA was added. Samples were vigorously vortexed twice for 30 sec (kept on ice in between). 750 µl from the liquid phase was transferred into a fresh Eppendorf tube. Samples were centrifuged for 10 min (20000 x g, 4 °C). The pellet was resuspended in 300 µl TCA-Laemmli buffer and boiled for 10 min at 100 °C.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References

Trump et al.: "Commentary on: "Enzalutamide monotherapy in hormone-naive prostate cancer: primary analysis of an open-label, single-arm, phase 2 study." Tombal B, Borre M, Rathenborg P, Werbrouck P, Van Poppel H, ..." in: Urologic oncology, (2015) (PubMed).

Yoshida, Bacal, Desmarais, Padioleau, Tsaponina, Chabes, Pantesco, Dubois, Parrinello, Skrzypczak, Ginalski, Lengronne, Pasero: "The histone deacetylases sir2 and rpd3 act on ribosomal DNA to control the replication program in budding yeast." in: Molecular cell, Vol. 54, Issue 4, pp. 691-7, (2014) (PubMed).

Target Details

Target: Ribonucleotide Reductase Inhibiting Protein SML1

Background: Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.

Molecular Weight: expected: 11.83 kDa, apparent: 11-12 kDa

Gene ID: 854945