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CD8 antibody (FITC)

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Antigen
Reactivity
Human, Monkey
558
165
52
25
23
23
22
19
17
17
14
12
12
11
11
10
8
6
5
5
3
2
2
1
1
1
Host
Mouse
651
159
73
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
147
107
76
39
16
14
12
10
10
9
9
8
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
711
160
112
108
81
76
73
54
53
26
16
12
9
6
4
4
3
3
3
2
1
1
1
1
1
1
1
Options
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Immunogen Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1
Specificity This antibody recognises the CD8 cell surface glycoprotein.
Purification Ion Exchange Chromatography.
Background CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Nelson, Lever, Dean, Savage, Salguero, Pearce, Stevens, Simpson: "Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus)." in: Journal of medical microbiology, Vol. 59, Issue Pt 9, pp. 1107-13, 2010 (PubMed).

Kap, van Meurs, van Driel, Koopman, Melief, Brok, Laman, t Hart: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009 (PubMed).

Gibbings, Marcet-Palacios, Sekar, Ng, Befus: "CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses." in: BMC immunology, Vol. 8, pp. 12, 2007 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989 (PubMed).