MEK2 antibody (C-Term)

Catalog No. ABIN6738527

The Rabbit Polyclonal anti-MEK2 antibody is suitable to detect MEK2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IHC (p).

Quick Overview for MEK2 antibody (C-Term) (ABIN6738527)

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MEK2 Antibody

Binding Specificity: C-Term

Specificity: Human MAP2K2 / MEK2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig, Chicken (92%) Guinea pig (91%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MAP2K2 (P36507, NP_109587). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Galago, Marmoset, Elephant, Dog, Bovine, Bat, Horse, Pig, Turkey, Chicken (92%), Guinea pig, Zebra finch (91%), Opossum (85%), Mouse, Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MAP2K2 / MKK2 / MEK2

Background: Name/Gene ID: MAP2K2
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MAPKK 2, MKK2, MEK 2, MEK2, MAPKK2, PRKMK2

Gene ID: 5605

NCBI Accession: NP_109587

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling