AP2B1 antibody (AA 497-546)

Catalog No. ABIN6738853

The Rabbit Polyclonal anti-AP2B1 antibody is suitable to detect AP2B1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Monkey, Pig, Xenopus laevis and Bat. It has been validated for WB.

Quick Overview for AP2B1 antibody (AA 497-546) (ABIN6738853)

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Monkey, Pig, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AP2B1 Antibody

Binding Specificity: AA 497-546

Specificity: Human AP2B1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa497-546 of human AP2B1 (P63010, NP_001025177). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%), Drosophila, Beetle (92%), Slime mold (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: AP2B1

Background: Name/Gene ID: AP2B1

Synonyms: AP2B1, Adaptin, beta 2 (beta), ADTB2, AP-2 complex subunit beta, AP2-BETA, Beta-adaptin, CLAPB1, AP105B, Beta-2-adaptin

Gene ID: 163

NCBI Accession: NP_001025177

UniProt: P63010

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation