IGFBPL1 antibody (AA 18-270)

Catalog No. ABIN327188

Product Details anti-IGFBPL1 Antibody

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Binding Specificity: AA 18-270

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBPL1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Recognizes mouse IGFBP-L1. (aa 18-270) Cross-reactivity: rhIGFBP-L1:10 % and rmIGFBP-7 less than 1 %. Species sequence homology: Rat 96 %, human 79 %.

Purification: Immunoaffinity purified

Immunogen: NS0-derived recombinant rat rmIGFBP-L1.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western blot, Immunohistochemistry, and Direct ELISA. Western Blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Immunohistochemistry: 10 μg/mL detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4 % PFA+14 % picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After. blocking, sections were incubated with primary antibody for 3 hours at RT followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, sections were. washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 0.5 mL sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS.

Target Details for IGFBPL1

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Alternative Name: IGFBPL1 (IGFBPL1 Products)

Synonyms: IGFBP-RP4 antibody, IGFBPRP4 antibody, bA113O24.1 antibody, 2810011G06Rik antibody, 2810453O06Rik antibody, IGFBPL antibody, insulin like growth factor binding protein like 1 antibody, insulin-like growth factor binding protein-like 1 antibody, IGFBPL1 antibody, Igfbpl1 antibody

Background: Name/Gene ID: IGFBPL1

Synonyms: IGFBPL1, BA113O24.1, IGFBP-RP4, IGFBP-related protein 10, IGFBPRP4

Gene ID: 347252

UniProt: Q8WX77