ADP-Ribosylation Factor 1/2/3/4 (ARF1/2/3/4) antibody

Antigen: ADP-Ribosylation Factor 1/2/3/4 (ARF1/2/3/4)

Synonyms: A2MP, ARD1, ARFD1, RNF46, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, ARF1GAP, MGC39924, HRIHFB2281, MGC72830, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, Arf1gap, AI115377, Arf GAP1, MGC53624, arf2, MGC52628, arf-1, MGC52573, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, TRIM23, ADP-RIBOSYLATION FACTOR, ARF1A1C, T8I13.1, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334352

Additional Information

Alternative name: ARF1, 2, 3, 4

Gene ID: 375, 376, 377

Immunogen: C-EGLDWLSNQLRNQK

Description: All names and symbols: ARF1, ADP-ribosylation factor 1. Official Symbol: ARF1. RefSeq number(s): NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1

Specificity: This antibody is expected to recognize all four reported isoforms. Human, Mouse, Rat, Dog 181 aa

Synonyms: A2MP, ARD1, ARFD1, RNF46, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, ARF1GAP, MGC39924, HRIHFB2281, MGC72830, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, Arf1gap, AI115377, Arf GAP1, MGC53624, arf2, MGC52628, arf-1, MGC52573, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, TRIM23, ADP-RIBOSYLATION FACTOR, ARF1A1C, T8I13.1, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334352 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Dubois, Paleotti, Mironov et al.: "Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics." in: Nature cell biology, Vol. 7, Issue 4, pp. 353-64, 2005 (PubMed).