Betaine--Homocysteine S-Methyltransferase (BHMT) antibody

Antigen: Betaine--Homocysteine S-Methyltransferase (BHMT)

Synonyms: MGC46866, MGC117976, MGC123027, wu:fb53h01, wu:fb63c08, wu:fj64d01, zgc:123027, hm:zehn2153, BHMT, MGC137173, DKFZp469D1732, bhmt

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334358

Additional Information

Alternative name: BHMT

Gene ID: 635, 12116 (mouse), 81508 (rat)

Immunogen: C-EQQLKELFEKQK

Description: All names and symbols: BHMT, betaine-homocysteine methyltransferase. Official Symbol: BHMT. RefSeq number(s): NP_001704.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism, Amino Acids, Enzymes

Restrictions: For Research Use only

Images

ABIN334358 (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BHMT RC203148) and probed with ABIN334358 (mock transfection in first lane), tested by Origene.

Publications

Publications: Collinsova, Strakova, Jiracek et al.: "Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice." in: The Journal of nutrition, Vol. 136, Issue 6, pp. 1493-7, 2006 (PubMed).