Chromodomain Helicase DNA Binding Protein 1 (CHD1) (internal) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 1 (CHD1)

Synonyms: DKFZp686E2337, CHD1, CHD-Z, Chd1, AI851787, AW555109, MGC141554, 4930525N21Rik

Epitope: internal

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334373

Additional Information

Alternative name: CHD1 (internal)

Gene ID: 1105, 12648 (mouse), 308215 (rat)

Immunogen: C-NGKDHRDWDHYKQD

Description: All names and symbols: CHD1, chromodomain helicase DNA binding protein 1, DKFZp686E2337. Official Symbol: CHD1. RefSeq number(s): NP_001261.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 110kDa and 30kDa in lysates of cell line HeLa after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 197kDa according to NP_001261.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Eissenberg, Lee, Schneider et al.: "The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase." in: Nature structural & molecular biology, Vol. 14, Issue 4, pp. 344-6, 2007 (PubMed).