Cysteine and Glycine-Rich Protein 3 (Cardiac LIM Protein) (CSRP3) antibody

Antigen: Cysteine and Glycine-Rich Protein 3 (Cardiac LIM Protein) (CSRP3)

Synonyms: CLP, MLP, CRP3, LMO4, CMD1M, CMH12, MGC14488, MGC61993, MMLP, csrp3, csrp2, MGC89085, zgc:103468, CSRP3

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334380

Additional Information

Alternative name: CSRP3

Gene ID: 8048, 13009 (mouse), 117505 (rat)

Immunogen: CGKSLESTNVTDKD

Description: All names and symbols: CSRP3, cysteine and glycine-rich protein 3 (cardiac LIM protein), CLP, CMD1M, CRP3, LMO4, MGC14488, MGC61993, MLP, LIM domain only 4, cardiac LIM domain protein, cardiac LIM protein, cysteine and glycine-rich protein 3, cysteine-rich protein 3, muscle LIM protein. Official Symbol: CSRP3. RefSeq number(s): NP_003467.1

Specificity: Tested Reactivity: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 20kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kDa according to Human NP_003467.1 and 20.8kDa according to Rat NP_476485.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN334380 (0.01µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zheng, Wen, Han: "hhLIM is a novel F-actin binding protein involved in actin cytoskeleton remodeling." in: The FEBS journal, Vol. 275, Issue 7, pp. 1568-78, 2008 (PubMed).