Ecto-NOX Disulfide-Thiol Exchanger 2 (ENOX2) antibody

Antigen: Ecto-NOX Disulfide-Thiol Exchanger 2 (ENOX2)

Synonyms: APK1, tNOX, COVA1, Cova1, AI314192, MGC32453, RGD1563643

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334390

Additional Information

Alternative name: ENOX2 / APK1

Gene ID: 10495

Immunogen: C-EKLKDDKLQVEK

Description: All names and symbols: ecto-NOX disulfide-thiol exchanger 2, RP5-875H3.1, APK1, COVA1, tNOX, APK1 antigen, cytosolic ovarian carcinoma antigen 1. Official Symbol: ENOX2. RefSeq number(s): NP_006366.2, NP_872114.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_006366.2, NP_872114.1). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 60kDa and 37kDa in Human Heart lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 66.6kDa and 70.1kDa according to NP_006366.2 and NP_872114.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Chueh, Wu, Morre et al.: "tNOX is both necessary and sufficient as a cellular target for the anticancer actions of capsaicin and the green tea catechin (-)-epigallocatechin-3-gallate." in: BioFactors (Oxford, England), Vol. 20, Issue 4, pp. 235-49, 2005 (PubMed).