EPH Receptor B2 (EPHB2) antibody

Antigen: EPH Receptor B2 (EPHB2)

Synonyms: DRT, ERK, CAPB, Hek5, PCBC, EPHT3, Tyro5, MGC87492, RGD1564232, EPHB2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334391

Additional Information

Alternative name: EPHB2 / DRT / Nuk

Gene ID: 2048, 13844 (mouse)

Immunogen: C-QKDRNHRPKFGQ

Description: All names and symbols: EPHB2, DRT, EPH receptor B2, Cek5, Qek5, Sek3, ETECK, Prkm5, EPHT3, ERK, Hek5, MGC87492, Tyro5, developmentally-regulated eph-related tyrosine kinase, elk-related tyrosine kinase, eph tyrosine kinase 3, ephrin receptor EphB2. Official Symbol: EPHB2. RefSeq number(s): NP_004433.2, NP_059145.2

Specificity: This antibody is expected to recognise both reported isoforms ( NP_059145.2 and NP_004433.2). Human, Mouse, Rat, Dog 986 aa, 987 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Approx 60kDa band observed in Human Lung and Placenta lysates (calculated MW of 110kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem. 2007 Apr 10, PMID: 17428795). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334391 (1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Cramer, Cerretti, Siddiqui: "EphB2 regulates axonal growth at the midline in the developing auditory brainstem." in: Developmental biology, Vol. 295, Issue 1, pp. 76-89, 2006 (PubMed).