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Antibodies
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anti-Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation...
Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (ERCC1) antibody
| Antigen |
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| Synonyms |
UV20, COFS4, Ercc-1, ercc1, MGC53204, ERCC1, MGC140762 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (96), Rat (Rattus) (21), Dog (Canine) (20), Mouse (Murine) (20), Pig (Porcine) (17), Cow (Bovine) (16), Monkey (3), Horse (Equine) (2), Sheep (Ovine) (2), Chicken (1), Rabbit (1)
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Application
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Western Blotting (WB) (82), Flow Cytometry (FACS) (58), Immunofluorescence (IF) (51), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), ELISA (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN334392 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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ERCC1
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Gene ID
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2067
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Immunogen
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DPGKDKEGVPQPS-C
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Description
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All names and symbols: ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein. Official Symbol: ERCC1. RefSeq number(s): NP_973730.1, NP_001974.1
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Specificity
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This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1). Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Cancer, DNA/RNA
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Restrictions
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For Research Use only
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Publications
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Zhang, Liu, Li et al.: "Double-strand breaks induce homologous recombinational repair of interstrand cross-links via cooperation of MSH2, ERCC1-XPF, REV3, and the Fanconi anemia pathway." in: DNA repair, Vol. 6, Issue 11, pp. 1670-8, 2007 (PubMed).
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Alternatives for antigen "Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (ERCC1)", type "Antibodies"
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Hosts
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Mouse (76), Rabbit (37), Goat (2)
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Reactivities
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Human (96), Rat (Rattus) (21), Dog (Canine) (20), Mouse (Murine) (20), Pig (Porcine) (17), Cow (Bovine) (16), Monkey (3), Horse (Equine) (2), Sheep (Ovine) (2), Chicken (1), Rabbit (1)
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Applications
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Western Blotting (WB) (82), Flow Cytometry (FACS) (58), Immunofluorescence (IF) (51), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), ELISA (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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transcript variant 2 (16)
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Alternatives 
