ERCC1 antibody (AA 191-281)

Catalog No. ABIN6939333

This Mouse Monoclonal antibody specifically detects ERCC1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ERCC1 antibody (AA 191-281) (ABIN6939333)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: ERCC1-2318

Product Details anti-ERCC1 Antibody

Binding Specificity: AA 191-281

Purpose: ERCC1 / RAD10 (Tumor Progression Marker) Antibody

Specificity: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: HeLa, HepG2 cells. Tonsil or Prostate.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1

Background:

COFS4, DNA excision repair protein ERCC1, ERCC1, Excision repair cross complementing 1, RAD10, UV20

HeLa or HepG2 cells. Human tonsil or prostate.

Molecular Weight: 110kDa

Gene ID: 2067, 435981

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response