HEXIM1 antibody (Hexamethylene Bis-Acetamide Inducible 1) (C-Term)

Details for Product anti-HEXIM1 Antibody No. ABIN334418, Supplier: Log in to see
Antigen
  • HEXIM1
  • fd44h02
  • hexim
  • wu:fd44h02
  • zgc:162976
  • CLP1
  • EDG1
  • HIS1
  • MAQ1
  • 7330426E13Rik
  • CLP-1
  • Clp1
  • Clp-1
  • hexamethylene bis-acetamide inducible 1
  • HEXIM1
  • LOC100339675
  • hexim1
  • Hexim1
Epitope
C-Term
16
15
15
11
9
8
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
123
77
41
6
5
4
3
3
3
2
2
1
1
1
1
Host
Goat
94
18
16
2
1
Clonality
Polyclonal
Conjugate
This HEXIM1 antibody is un-conjugated
6
6
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
108
65
44
23
22
13
9
6
6
4
2
2
Options
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Immunogen Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.
Blocking Peptide Blocking peptide for this product available: ABIN369204
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HEXIM1 (HEXIM1 Antibody Abstract)
Background Synonyms: HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1, HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, EDG1, FLJ13562
Gene ID 10614, 192231
NCBI Accession NP_006451
Research Area Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65 kDa band observed in HeLa cell lysate and using recombinant HEXIM1 protein. Data kindly provided by David Price and Jeff Cooper, University of Iowa, USA. We find an approx. 40 kDa band in Human Heart lysates (calculated MW of 40.6 kDa according to NP_006451.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-HEXIM1 antibody (Hexamethylene Bis-Acetamide Inducible 1) (C-Term) (ABIN334418) ABIN334418 (0.3µg/ml) staining of 1) HeLa cell lysate and 2) recombinant HEXIM1 (3ng)...
Background publications Schulte, Czudnochowski, Barboric, Schönichen, Blazek, Peterlin, Geyer: "Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24968-77, 2005 (PubMed).