IKAROS Family Zinc Finger 1 (IKZF1) antibody

Antigen: IKAROS Family Zinc Finger 1 (IKZF1)

Synonyms: IK1, LYF1, hIk-1, IKAROS, PRO0758, ZNFN1A1, Hs.54452, RGD1562979, ikaros, znfn1a1, MGC152552, ik1, lyf1, hik-1, pro0758, hs.54452, MGC108252

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334424

Additional Information

Alternative name: IKZF1 / IKAROS

Gene ID: 10320, 22778 (mouse)

Immunogen: C-DLLRAASENSQDALR

Description: All names and symbols: IKZF1, IKAROS family zinc finger 1 (Ikaros) , Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1 , CLL-associated antigen KW-6, Ikaros (zinc finger protein), zinc finger protein, subfamily 1A, 1 (Ikaros) . Official Symbol: IKZF1. RefSeq number(s): NP_006051.1

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334424 (0.03µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Mullighan, Goorha, Radtke et al.: "Genome-wide analysis of genetic alterations in acute lymphoblastic leukaemia." in: Nature, Vol. 446, Issue 7137, pp. 758-64, 2007 (PubMed).