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Antibodies
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anti-Nucleoporin 62kDa (NUP62) antibody
Nucleoporin 62kDa (NUP62) antibody
| Antigen |
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| Synonyms |
p62, IBSN, SNDI, MGC841, FLJ20822, FLJ43869, DKFZp547L134, NUP62, DKFZp459M091 |
| Clonality |
Polyclonal |
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Host
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Application
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Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), Western Blotting (WB) (16), ELISA (8), Enzyme Immunoassay (EIA) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN334450 |
| Quantity |
100 ug |
| Price |
369.20 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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NUP62
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Gene ID
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23636
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Immunogen
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C-RKEQERSFRITFD
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Description
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All names and symbols: NUP62, p62, MGC841, FLJ20822, DKFZp547L134, nucleoporin 62kDa, nuclear pore glycoprotein p62. Official Symbol: NUP62. RefSeq number(s): NP_036478.2, NP_057637.2, NP_714940.1, NP_714941.1
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Specificity
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All four variants represent identical protein. Human 522 aa,
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.3kDa according to NP_036478.2, NP_057637.2, NP_714940.1 and NP_714941.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Carmo-Fonseca, Kern, Hurt: "Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization." in: European journal of cell biology, Vol. 55, Issue 1, pp. 17-30, 1991 (PubMed).
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Alternatives for antigen "Nucleoporin 62kDa (NUP62)", type "Antibodies"
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Hosts
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Rabbit (25), Mouse (5), Hamster (3), Goat (2)
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Reactivities
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Human (30), Rat (Rattus) (23), Mouse (Murine) (20), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (16), Horse (Equine) (15), Sheep (Ovine) (2)
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Applications
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Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), Western Blotting (WB) (16), ELISA (8), Enzyme Immunoassay (EIA) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term E507 (1), C-Term Y422 (1)
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Alternatives 
