U4/U6 Small Nuclear Ribonucleoprotein Prp31 (PRPF31) antibody

Antigen: U4/U6 Small Nuclear Ribonucleoprotein Prp31 (PRPF31)

Synonyms: RP11, PRP31, NY-BR-99, DKFZp566J153, AW554706, 1500019O16Rik, 2810404O06Rik, prpf31, embryo defective 1220, T13D8.6, T13D8_6

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334465

Additional Information

Alternative name: PRPF31

Gene ID: 26121, 68988 (mouse)

Immunogen: KELGNSLDKCKNNEN

Description: All names and symbols: PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast) . Official Symbol: PRPF31. RefSeq number(s): NP_056444.2

Specificity: Human, Mouse, Rat, Dog 499 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4kDa according to NP_056444.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA

Restrictions: For Research Use only

Images

ABIN334465 (1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yuan, Kawada, Havlioglu et al.: "Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 3, pp. 748-57, 2005 (PubMed).