Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) antibody

Antigen: Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1)

Synonyms: OF, PU.1, SFPI1, SPI-1, SPI-A

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334471

Additional Information

Alternative name: PU.1

Gene ID: 6688, 366126 (rat)

Immunogen: C-DLYQRQTHEYY

Description: All names and symbols: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1. Official Symbol: SPI1. RefSeq number(s): NP_001074016.1, NP_003111.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2). Human, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30,275(26):19552-9., PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334471 (0.03µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Uvarov, Ludwig, Markkanen et al.: "Upregulation of the neuron-specific K+/Cl- cotransporter expression by transcription factor early growth response 4." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 52, pp. 13463-73, 2006 (PubMed).