Ring Finger Protein 213 (RNF213) (C-Term) antibody

Antigen: Ring Finger Protein 213 (RNF213)

Synonyms: C17orf27, KIAA1554, KIAA1618, RNF213

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN334480

Additional Information

Alternative name: RNF213 / C17orf27 (C Terminus)

Gene ID: 57674

Immunogen: C-KTAAVLKWNRE

Description: All names and symbols: RNF213, C17orf27, ring finger protein 213, chromosome 17 open reading frame 27, KIAA1554. Official Symbol: RNF213. RefSeq number(s): NP_065965.3

Specificity: Human 3280 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney . Recommended concentration, 2.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334480 (2.5µg/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Publications

Publications: Zody, Garber, Adams et al.: "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." in: Nature, Vol. 440, Issue 7087, pp. 1045-9, 2006 (PubMed).