RNF213 antibody (Internal Region)

Catalog No. ABIN334481

The Goat Polyclonal anti-RNF213 antibody has been validated for ELISA and IHC. It is suitable to detect RNF213 in samples from Human.

Quick Overview for RNF213 antibody (Internal Region) (ABIN334481)

Target: RNF213 (Ring Finger Protein 213 (RNF213))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF213 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RNF213 Antibody

Binding Specificity: Internal Region

Purpose: RNF213 / C17orf27 (internal)

Sequence: KNPQTQTEER FRPQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KNPQTQTEERFRPQ, from the internal region of the protein sequence according to NP_065965.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 1.25 μg/mL.

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374 kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF213

Target: RNF213 (Ring Finger Protein 213 (RNF213))

Alternative Name: RNF213

Background: Ring finger protein 213, RNF213, C17orf27, chromosome 17 open reading frame 27, KIAA1554

Gene ID: 57674

NCBI Accession: NP_065965