s100a4 antibody (C-Term)

Catalog No. ABIN334485

Product Details anti-s100a4 Antibody

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: S100A4 / CAPL

Sequence: CNEFFEGFPD KQP

Specificity: Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CNEFFEGFPDKQP, from the C Terminus of the protein sequence according to NP_002952.1, NP_062427.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 13 kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7 kDa according to NP_002952.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-s100a4 antibody (ABIN334485)

Rasanen, Sriswasdi, Valiga, Tang, Zhang, Perego, Somasundaram, Li, Speicher, Klein-Szanto, Basu, Rustgi, Speicher, Herlyn: "Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3778-92, (2013) (PubMed).

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 (S100A4 Products)

Synonyms: 18A2 antibody, 42A antibody, CAPL antibody, FSP1 antibody, MTS1 antibody, P9KA antibody, PEL98 antibody, 42a antibody, Capl antibody, FSp1 antibody, Mts1 antibody, PeL98 antibody, metastasin antibody, pk9a antibody, S100A4 antibody, P9ka antibody, RNP9KA antibody, S100 calcium binding protein A4 antibody, S100 calcium-binding protein A4 antibody, S100A4 antibody, S100a4 antibody

Background: S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium pr

Gene ID: 6275, 20198, 24615

NCBI Accession: NP_002952, NP_062427

Pathways: S100 Proteins