Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) antibody

Antigen: Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1)

Synonyms: smk1, FLFL1, smk-1, PP4R3A, MSTP033, KIAA2010, BC064465, mKIAA2010, 1110034C04Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334493

Additional Information

Alternative name: SMEK1 / KIAA2010

Gene ID: 55671, 68734 (mouse), 314388 (rat)

Immunogen: C-EQQRERQDNPKLD

Description: All names and symbols: SMEK homolog 1, suppressor of mek1 (Dictyostelium) , KIAA2010 , FLFL1 , MSTP033 , smk-1 , smk1, SMEK homolog 1, suppressor of mek1. Official Symbol: SMEK1. RefSeq number(s): NP_115949.3

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334493 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Panowski, Wolff, Aguilaniu et al.: "PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans." in: Nature, Vol. 447, Issue 7144, pp. 550-5, 2007 (PubMed).