Suppressor of Cytokine Signaling 7 (SOCS7) antibody

Antigen: Suppressor of Cytokine Signaling 7 (SOCS7)

Synonyms: NAP4, NCKAP4, Nap4, C85125, 2310063P06Rik, Socs7, GB13951, MGC175115, zgc:175115, SOCS6, SOCS4, SOCS7, socs7, AI427843, AU015727, AU015859, MGC143730, MGC143731, 3110032M18Rik, A730004F22Rik, DKFZp686J1568

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334497

Additional Information

Alternative name: SOCS7

Gene ID: 30837

Immunogen: C-DAGRKPKLTRTQS

Description: All names and symbols: SOCS7, suppressor of cytokine signaling 7, NAP4, Nck, Ash and phospholipase C binding protein. Official Symbol: SOCS7. RefSeq number(s): NP_055413.1

Specificity: Human

Synonyms: NAP4, NCKAP4, Nap4, C85125, 2310063P06Rik, Socs7, GB13951, MGC175115, zgc:175115, SOCS6, SOCS4, SOCS7, socs7, AI427843, AU015727, AU015859, MGC143730, MGC143731, 3110032M18Rik, A730004F22Rik, DKFZp686J1568

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 60kDa band observed in lysates of Daudi (calculated MW of 63.0kDa according to NP_055413.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN334497 (1µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kremer, Adang, Macara: "Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7." in: Cell, Vol. 130, Issue 5, pp. 837-50, 2007 (PubMed).