TAR DNA Binding Protein (TARDBP) antibody

Antigen: TAR DNA Binding Protein (TARDBP)

Synonyms: ALS10, TDP-43, Tdp43, C85084, 1190002A23Rik, fb77f02, fc52g10, wu:fb77f02, wu:fc52g10, tardbp, MGC53141, TARDBP, TDP43, DKFZp459I2127, MGC129120

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334503

Additional Information

Alternative name: TARDBP

Gene ID: 23435, 230908 (mouse)

Immunogen: Peptide with sequence C-HISNAEPKHNSNRQ, from the internal region of the protein sequence according to NP_031401.1

Description: All names and symbols: TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43. Official Symbol: TARDBP. RefSeq number(s): NP_031401.1

Specificity: Expected from sequence similarity: Human, Mouse, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 45kDa band observed in Human lysates of cell line Jurkat (calculated MW of 44.7kDa according to NP_031401.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines

Restrictions: For Research Use only

Images

ABIN334503 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zhang, Xu, Dickey et al.: "Progranulin mediates caspase-dependent cleavage of TAR DNA binding protein-43." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 39, pp. 10530-4, 2007 (PubMed).