L-Ascorbate Peroxidase (APX) antibody

Antigen: L-Ascorbate Peroxidase (APX)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: Western Blotting (WB)

Catalog no.: ABIN334581

Additional Information

Immunogen: synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490)and sAPX (At4g08390) protein sequences, coupled to BSA. Five out of twelveamino acids are also identical with cAPX1 (At1g07890), cPX2 (At3g09640) andpAPX (At4g35000)

Format: Lyophilized

Description: APX plays a key role in plant antioxidant system by reducing hydrogen peroxideto water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol(cAPX) and peroxisome (pAPX).

Specificity: Predicted reactivity: N. tabacum thylakoid-bound APX, stromal APX, O.sativa probable L-ascorbateperoxidase 6 chloroplastic, S. lycopersicum thylakoid-bound APX, S. oleraceastromal APX, thylakoid-bound.

Application Details

Application Notes: Recommended Dilution 1: 2000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

5 to 20 μg of total leaf protein from Arabidopsis thaliana (left panel) and chloroplast fractions (thylakoids and soluble, right panel) was separated on 15% polyacrylamide gel with 6M urea and blotted on PVDF. Filters were blocked 1h with 5% BSA, incubated with anti-APX antibody (1: 2000, 1h) followed by incubation with secondary HRP-coupled anti rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL. ABIN334581 is reactive to thylakoid (tAPX, 38 kDa), stromal (sAPX, 33 kDa), peroxisomal (pAPX, 31 kDa) and cytoplasmic (cAPX1 + cAPX2, 25 kDa) forms of ascorbate peroxidases.

Publications

Publications: Kangasjaervi, Lepistoe, Haennikaeinen et al.: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." in: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, 2008 (PubMed).

Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." in: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, 2008 (PubMed).

Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, 2010 (PubMed).