Ewing Sarcoma Breakpoint Region 1 (EWSR1) antibody

Antigen: Ewing Sarcoma Breakpoint Region 1 (EWSR1)

Synonyms: EWS, bK984G1.4, Ews, Ewsh, AU018891, MGC112874, EWSR1, fc04c01, wu:fc04c01, MGC179351, DKFZp459K1116

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN335135

Additional Information

Alternative name: EWS / EWSR1

Gene ID: 2130, 14030 (mouse), 289752 (rat)

Immunogen: C-TSYDQSSYSQQNTYG

Description: All names and symbols: EWSR1, EWS, Ewing sarcoma breakpoint region 1, EWS/FLI fusion protein, EWSR1/ZNF384 fusion protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, bK984G1.4 (Ewing sarcoma breakpoint region 1 protein), extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion protein. Official Symbol: EWSR1. RefSeq number(s): NP_053733.1, NP_005234.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_005234.1, NP_053733.1). Human, Mouse, Rat, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 90kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB09167 (0.1µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.