NudE Nuclear Distribution Gene E Homolog (A. Nidulans)-Like 1 (NDEL1) antibody

Antigen: NudE Nuclear Distribution Gene E Homolog (A. Nidulans)-Like 1 (NDEL1)

Synonyms: EOPA, NUDEL, MITAP1, DKFZp451M0318

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN335149

Additional Information

Alternative name: NDEL1

Gene ID: 81565, 83431 (mouse), 170845 (rat)

Immunogen: CRNFAKDQASRKSY

Description: All names and symbols: NDEL1, nudE nuclear distribution gene E homolog (A. nidulans)-like 1, DKFZp451M0318, EOPA, MITAP1, NUDEL, NUDE-like protein, endooligopeptidase A, mitosin-associated protein MITAP1, nudE nuclear distribution gene E homolog like 1. Official Symbol: NDEL1. RefSeq number(s): NP_001020750.1, NP_110435.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_001020750.1, NP_110435.1) Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 38+39kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 37.0kDa according to NP_001020750.1 and of 38.4kDa according to NP_110435.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB09175 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.