Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) antibody

Antigen: Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2)

Synonyms: BXR, PAR, PRR, PXR, SAR, SXR, ONR1, PAR1, PAR2, PARq, PXR1, mPXR, PXR.1, PXR.2, MGC108643

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN335159

Additional Information

Alternative name: PXR / NR1I2

Gene ID: 8856

Immunogen: EQFAITLKSYIECNR

Description: All names and symbols: PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor. Official Symbol: NR1I2.

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB09172 (1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.