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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Vimentin (VIM) antibody
|Synonyms||FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime|
Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken, Goat, Dog (Canine), Monkey, Zebrafish (Danio rerio)
Alternatives Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|5 references available|
|Price||607.20 $ Plus shipping costs $45.00|
|Immunogen||RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.|
|Description||Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.|
|Specificity||Human, mouse, rat, hamster, chicken, goat, canine and monkey.|
|Application Notes||RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.|
|Storage||Store at 4 o C, or in small aliquots at -20 o C.|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Restrictions||For Research Use only|
|anti-Vimentin (VIM) antibody anti-Vimentin (VIM) antibody (Image 2)|
Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).
Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).
Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).
Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988 (PubMed).
Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).