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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal (V9)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Rat (Rattus), Chicken, Potoroo
Application
Alternatives Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
5 references available
Catalog no. ABIN335382
Quantity 100ug  (Variants)
Price 543.64 $   Plus shipping costs $35.00
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anti-Vimentin (VIM) antibody

Additional Information
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Alternative name Vimentin
Immunogen V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Format Purified
Isotype IgG1  (Matching secondary antibodies)
Clone V9
Description Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Specificity Human, rat, chicken and potoroo.

Application Details
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Application Notes V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Storage Store at 4 o C, or in small aliquots at -20 o C.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Restrictions For Research Use only

Publications
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Publications Toelle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985 (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984 (PubMed).

Brussee, Smit, Koopman et al.: "Interrupter resistance and wheezing phenotypes at 4 years of age." in: American journal of respiratory and critical care medicine, Vol. 169, Issue 2, pp. 209-13, 2004 (PubMed).

Alternatives
Application Details Publications Images Secondary Antibodies Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Rabbit (95), Mouse (73), Chicken (9), Sheep (3), Guinea Pig (2), Goat (1)
Reactivities Human (156), Rat (Rattus) (65), Mouse (Murine) (60), Chicken (31), Cow (Bovine) (28), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Fish (1), Potoroo (1), Primate (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (105), Immunofluorescence (IF) (74), Flow Cytometry (FACS) (56), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (56), Immunohistochemistry (IHC) (54), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (16), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
Conjugates FITC (10), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitopes pSer82 (21), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)