Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M) (AA 355-366) antibody

Catalog No. ABIN337024

This Goat Polyclonal antibody specifically detects Eukaryotic Translation Initiation Factor 3, Subunit M in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Bat, Monkey and Pig.

Quick Overview for Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M) (AA 355-366) antibody (ABIN337024)

Target: Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Bat, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 355-366

Specificity: Human EIF3M / PCID1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%) Hamster, Opossum, Platypus, Xenopus (92%) Turkey, Chicken (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NAWKQNLNKVKN from the C-terminus of human EIF3M / PCID1 (NP_006351.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%), Hamster, Opossum, Platypus, Xenopus (92%), Turkey, Chicken (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Eukaryotic Translation Initiation Factor 3, Subunit M (EIF3M)

Alternative Name: EIF3M / PCID1

Background: Name/Gene ID: EIF3M

Synonyms: EIF3M, B5, B5 receptor, Dendritic cell protein, GA17, HFLB5, Fetal lung protein B5, Hfl-B5, TANGO7, PCID1

Gene ID: 10480

NCBI Accession: NP_006351

UniProt: Q7L2H7

Pathways: Ribonucleoprotein Complex Subunit Organization