Merlin antibody (AA 526-536)

Catalog No. ABIN337064

The Goat Polyclonal anti-Merlin antibody is suitable to detect Merlin in samples from Human and Zebrafish (Danio rerio). It has been validated for ELISA, IHC and IHC (p).

Quick Overview for Merlin antibody (AA 526-536) (ABIN337064)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivity: Human, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Merlin Antibody

Binding Specificity: AA 526-536

Specificity: Zebrafish NF2.

Predicted Reactivity: Percent identity by BLAST analysis: Pufferfish, Zebrafish (100%) Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Drosophila, Ant, Paramecium (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKSKHLQDQLK from an internal region of zebrafish NF2 (NP_998116.1). Percent identity by BLAST analysis: Pufferfish, Zebrafish (100%), Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Drosophila, Ant, Paramecium (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Zebrafish

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: NF2 / Merlin

Background: Name/Gene ID: NF2

Synonyms: NF2, ACN, BANF, Moesin-ezrin-radixin like, Neurofibromin 2 (merlin), Neurofibromin-2, SCH, Merlin, Schwannomerlin, Schwannomin

Gene ID: 4771

NCBI Accession: NP_998116

UniProt: P35240

Pathways: Cell-Cell Junction Organization